LC-MS Guide is a practical resource for anyone who works with liquid chromatography and mass spectrometry — in pharmaceutical, environmental, clinical, food and academic laboratories.
Most chromatography problems are not mysterious. A drifting baseline, a tailing peak, a sudden loss of sensitivity — each has a limited set of causes that can be worked through systematically. The articles here are written to do exactly that: identify the symptom, walk through the likely causes in order of probability, and give you something concrete to test at the instrument.
What you will find here
- Troubleshooting guides — structured workflows for HPLC, UHPLC and LC-MS problems, from baseline drift to ion suppression and carryover.
- Column guides — which stationary phases are actually used for which analytes and matrices, based on the published literature rather than marketing claims.
- Method development — mobile phases, buffers, pH, additives and the decisions that shape a robust method.
- Fundamentals — the concepts behind the practice, written for people who are new to the technique or returning to it.
How the content is written
Recommendations are backed by real, linked sources — peer-reviewed publications and manufacturer documentation — and never by invented numbers. Where the literature is thin or the evidence is mixed, that is stated plainly rather than smoothed over. Links to manufacturers are provided for convenience only; this site is independent and not affiliated with any column or instrument vendor.
Found an error, or have a problem you would like covered? Get in touch — corrections and topic suggestions are genuinely welcome.